24 hour PCR /STEC
24-hour rapid pathogen testing – PCR Solutions
Advanced Laboratory Testing are delighted to offer 24-hour rapid pathogen testing solutions using PCR Technology.
DETECTION OF SALMONELLA SPP USING REAL-TIME PCR
MTM037 DETECTION OF SALMONELLA SPP USING REAL-TIME PCR is based upon BIORAD iQ-Check™ Salmonella II Kit and can obtain negative results in less than 24 hours.
• Next day results for all food products.
• Validated on a wide range of food products including dairy, meat, and produce.
• >98% specificity.
• INAB Accredited.
DETECTION OF LISTERIA SPP USING REAL-TIME PCR
MTM038 DETECTION OF LISTERIA SPP USING REAL-TIME PCR is based upon BIORAD iQ-Check™ Listeria and can obtain negative results in less than 24 hours.
The iQ-Check Listeria spp. test is based on gene amplification and Real-time PCR detection. It uses primers and a DNA probe specific to Listeria spp. Following the pre-enrichment step in the specific LSB broth, bacterial lysis releases bacterial DNA. The amplification and detection steps are then performed in a thermal cycler. At the end of the reaction, fluorescence is emitted and measured directly by the thermal cycler. The software associated with the instrument analyzes the results, which are displayed in the form of curves to be interpreted.
ALT are INAB Accredited for 21 sample matrices.
SHIGA TOXIN-PRODUCING ESCHERICHIA COLI (STEC)
Testing solution using iQ-Check™ STEC Real Time PCR system.
E. coli bacteria are normal flora in human and animal intestines and are usually harmless.
However, some strains can cause diseases to humans. Among them, Shiga toxin-producing Escherichia coli (STEC) are known to be highly pathogenic to humans. They can lead to hemorrhagic colitis and hemolytic uremic syndrome (HUS). STECs are defined by the presence of the stx1 or stx2 (Shiga toxin genes) in their genome. The eae (intimin) gene is an additional virulence marker.
The most known of these STEC strains is E. coli O157:H7 for which there is a “zero tolerance” policy in North America. Such policy will be implemented by FSIS for non-O157 STEC as well, in the meat sector. Outbreaks are commonly associated with the consumption of raw meat, but also with dairy products and more recently with produce. In the context of the ISO or the FSIS guidelines, a sample positive for both stx1/2 and eae targets will have to be tested for the presence of the 4 or 6 major E. coli serogroup: O26, O45, O103, O111, O121 and O145 in addition to E. coli O157:H7.
Our in-house Microbiology experts currently operate an STEC testing solution using an INAB Accredited iQ-Check™ STEC Real Time PCR system which is able to turnaround results within 24 hours.
MTM041A iQ-Check™ STEC VirX: Initial screening for the virulence genes in a single well. 24 hours to result.
MTM041B iQ-Check™ STEC SerO: Search for the 7 major serogroups (O26, O45, O103, O111, O121, O145 and O157:H7). 3 hours to result after initial screening.
MTM041C Confirmation of Detected serogroups. 72 hours to result.
iQ-Check STEC VirX is validated by AOAC Research Institute under the Performance Tested Method Program for detection of stx1/stx2 and eae in raw beef trim. Certificate number: 121203.
iQ-Check STEC SerO is validated by AOAC Research Institute under the Performance Tested Method Program for detection of Escherichia coli O157:H7, O26, O45, O103, O111, O 121 and O145 in raw beef trim. Certificate number: 121203.
Methods compliant with the ISO 13136 standard and USDA-FSIS Technical Guidelines.
iQ-Check STEC VirX has been granted a No Objection Letter from the United States Department of Agriculture Food Safety and Inspection Service (USDA FSIS) for raw beef trim.
iQ-Check STEC SerO has been granted a No Objection Letter from the United States Department of Agriculture.